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Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369)

Tris-Bis PAGE - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.com SEC-HPLC - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.com Standard Curve - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.com Functional Assay - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.com
This product is discontinued

Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) has been discontinued and is no longer available.

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  • Specifications

Name
Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Sequence
MIETYNQTSPRSAATGLPISMKIFMYLLTVFLITQMIGSALFAVYLHRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Tag
N-terminal monomeric Human Fc Tag
Conjugate

Unconjugated

Purity
>95% (by SDS-PAGE and HPLC).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Tris-Bis PAGE - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.comEnlarge Image

Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369)

Tris-Bis PAGE of Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) under reducing conditions confirms that purity is greater than 95%.
SEC-HPLC - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.comEnlarge Image

Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369)

SEC-HPLC analysis of Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) confirms that purity is greater than 95%.
Standard Curve - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.comEnlarge Image

Standard Curve - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369)

ELISA examination of immobilized Human CD40, His Tag, (1 µg/mL) binding Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369): EC50 =9.0ng/mL.
Functional Assay - Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) - Antibodies.comEnlarge Image

Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369)

Inhibition of the binding between Recombinant Human TRAP/CD40L Protein (N-terminal monomeric Human Fc Tag) (A331369) and RP00550B by addingserial dilutions of Anti-CD40 Antibody: IC50 = 2.0µg/mL.