Inhibition of the TWEAK-induced apoptosis in HT-29 human colon adenocarcinoma cells: ED50 = 2-12 µg/mL in the presence of 1 µg/mL recombinant human TWEAK. Immobilized Human TNFSF12 at 2 µg/mL bind Human TNFRSF12A in a functional ELISA with a linear range of 0.1-2.3 ng/mL. Inhibition of the the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells: ED50 = 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human TWEAKR/FN14 Protein (C-terminal Human Fc and His Tag) (A331389)
ELISA examination of immobilized Human TNFSF12 (2 µg/mL) binding Recombinant Human TWEAKR/FN14 Protein (C-terminal Human Fc and His Tag) (A331389): linear range = 0.1-2.3 ng/mL.
Recombinant Human TWEAKR/FN14 Protein (C-terminal Human Fc and His Tag) (A331389)
Inhibition of TWEAK-dependent cell proliferation for HUVEC (Human Umbilical Vein Endothelial Cells) with Recombinant Human TWEAKR/FN14 Protein (C-terminal Human Fc and His Tag) (A331389): ED50 = 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.
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