Immobilized protein supports the adhesion of U937 human histiocytic lymphoma cells (5 x 10E4 cells/well) are added to human VCAM1 coated plates (10µg/mL with 100 µL/well): approximately 80%-90% cells adhere after 1 hour at 37?. Immobilized protein in a functional ELISA.Immobilized APC anti-human CD106 Antibody at 1µg/mL (25 µL/well) can bind Human VCAM1 with a linear range of 0.46-16.9ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
CD106, INCAM-100, V-CAM 1, Vascular cell adhesion protein 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human VCAM-1 Protein (C-terminal His Tag) (A331404)
ELISA examination of immobilized APC anti-human CD106 Antibody (1µg/mL) binding Recombinant Human VCAM-1 Protein (C-terminal His Tag) (A331404): linear range = 0.46-16.9ng/mL.
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