ED50: 10 ng/ml, as determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces, corresponding to a specific activity of 100,000 IU/mg. In a 13 day CAM-assay sVEGFR-1 is able to inhibit VEGF stimulated sprouting of capillaries at 30 pM.
Purity
>90% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
1 mg/ml
Formulation
Lyophilized without additives.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
