Inhibition of the the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC): ED50 = typically 10-50 µg/mL in the presence of 10 ng/mL recombinant human VEGF165. Immobilized PE anti-human CD309 (VEGFR2) Antibody at 1 µg/mL (25 µL/well) can bind Human VEGFR2 in a functional ELISA with a linear range of 0.46-68.8 ng/mL.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human VEGF Receptor 2 Protein (C-terminal His Tag) (A331408)
ELISA examination of immobilized PE anti-human CD309 (VEGFR2) Antibody (1µg/mL) binding Recombinant Human VEGF Receptor 2 Protein (C-terminal His Tag) (A331408): linear range = 0.46-68.8 ng/mL.
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