Immobilized Recombinant Human VEGF121 (1 µg/mL) bind Recombinant Human VEGFR2 in a functional ELISA with a linear range of 4-10 ng/mL. Cell proliferation assay using HUVEC human umbilical vein endothelial cells: ED50 = 0.09-0.36 ng/mL. Recombinant Human VEGFA(40 ng/mL) and bFGF(50 ng/mL, Cat. RP01162) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells: pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium after 4 days.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
L-VEGF, Vascular endothelial growth factor A, long form, Vascular permeability factor, VEGF, VPF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)
SDS-PAGE of Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416) under reducing (R) and non-reducing (NR) conditions to show single bands at 17-22 kDa and 35-45 kDa, respectively.
Standard Curve - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)
ELISA examination of immobilized Recombinant Human VEGF121 (1 µg/mL) binding Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416): linear range = 4-10 ng/mL.
Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)
Cell proliferation assay using Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416) on HUVEC Human umbilical vein endothelial cells : ED50 = 0.09-0.36 ng/mL.
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