Recombinant Lambda Protein Phosphatase (Functional)
Applications
SDS-PAGE, Functional Studies
Applications
Lambda Protein Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine, tyrosine, and histidine residues in proteins. It should be noted that different proteins are dephosphorylated at different rates. Optimal reaction temperature is 30°C.
Expression System
Escherichia coli
Nature
Recombinant
Protein Length
Full length protein
Biologically Active
Yes
Biological Activity
~400,000 units/mg protein.
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-nitrophenyl phosphate per minute at 30°C.
Purity
> 95% (by SDS-PAGE).
Product Form
Liquid
Concentration
400 units/µl
Formulation
Supplied in 50mM HEPES, pH 7.5, with 100mM NaCl, 2mM Dithiothreitol, 0.1 mM MnCl2, 0.1mM EDTA, 50% Glycerol, and 0.01% Brij 35.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Lambda Protein Phosphatase (Functional) (A461)
Recombinant Lambda Protein Phosphatase (Functional)
Publishing research using Recombinant Lambda Protein Phosphatase (Functional) (A461)? Please let us know so that we can list the citation on this page.
