Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5mg/ml in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (eg 0.1% BSA , 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.