Biological Activity
Immobilized Human Activin A, No Tag (0.2 µg/mL) binds Mouse FSTL3, His Tag, in an ELISA: EC50 = 18.0 ng/mL. Mouse FSTL3, His Tag captured on CM5 Chip via anti-His antibody bind Human Activin A, No tag, in SPR assay (Biacore T200) with an affinity constant of 53.09 pM. Neutralization of the Activin-mediated inhibition on MPC11 cell proliferation: ED50: 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.