Immobilized Mouse IL1B (10 µg/mL) bind Human IL1R2 in a functional ELISA with a linear range of 0.03125-0.8036 µg/mL. Cell proliferation assay using D10.G4.1 mouse helper T cells: ED50 = 42.1-168.4 pg/mL; Specific activity =5.94×10^6-2.37×10^7units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
Catabolin, IL1B, IL1F2, Interleukin-1 beta
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795)
ELISA examination of immobilized Mouse IL1B (10 µg/mL) binding Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795): linear range = 0.03125-0.8036 µg/mL.
Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795)
Cell proliferation assay using Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795) on D10.G4.1 Mouse helper T cells : ED50 = 3.10-12.40 pg/mL; Specific activity = 8.06×10^7~3.23×10^8 units/mg..
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