Immobilized Mouse IL-17A/CTLA-8 (2 µg/mL) bind Mouse IL-17RA/CD217 in a functional ELISA with a linear range of 0.1-26 ng/mL. Induction of the IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFa: ED50 = 0.26-1.04 ng/mL; Specific activity =9.62×10^5~3.85×10^6 units/mg.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Mouse IL-17A Protein (C-terminal His Tag) (A331670)
ELISA examination of immobilized Mouse IL-17A/CTLA-8 (2 µg/mL) binding Recombinant Mouse IL-17A Protein (C-terminal His Tag) (A331670): linear range = 0.1-26 ng/mL.
Standard Curve - Recombinant Mouse IL-17A Protein (C-terminal His Tag) (A331670)
ELISA examination of Recombinant Mouse IL-17A Protein (C-terminal His Tag) (A331670) to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of TNFa (2 ng/mL): ED50 = 0.26-1.04 ng/mL; Specific activity = 9.62×10^5~3.85×10^6 units/mg.
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