Inhibition of cell proliferation for MC/9 2 mouse mast cells: ED50 = 2.8-11.3 pg/mL; Specific activity = 8.85×10^7~3.57×10^8 units/mg. Inhibition of cell proliferation for HT-2 mouse T cells: ED50 = 0.21-0.85ng/mL; Specific activity = 1.18×10^6~4.76×10^6 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Mouse IL-4 Protein (C-terminal His Tag) (A330909)
Cell proliferation assay using Recombinant Mouse IL-4 Protein (C-terminal His Tag) (A330909) on HT-2 Mouse T cells : ED50 = 0.21-0.85 ng/mL; Specific activity = 1.18 × 10^6~4.76 × 10^6 units/mg.
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