NF-?B reporter assay in HEK293 cells: ED50 = 40-200 pg/mL. Immobilized Mouse TNF-a (2µg/mL) binds Mouse TNFRSF1B in a functional ELISA with a linear range of 0.02-0.77ng/mL. Cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D: ED50 = 0.025-0.1 pg/mL.
Purity
>87% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)
ELISA examination of immobilized recombinant Mouse TNFa (2 µg/mL) binding Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883): linear range = 4-53 ng/mL.
Standard Curve - Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)
ELISA examination of immobilized Recombinant Mouse TNF-a (2 µg/mL) binding Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883): linear range = 0.02-0.77 ng/mL.
Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)
Cytotoxicity effects induced by Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883) in L-929 Mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D: ED50 = typically 3.55-14.2 pg/mL; Specific activity = 7.04×10^7~2.82×10^8 units/mg.
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