Immobilized Mouse TNF-a (2 µg/mL) bind Mouse TNFRSF1B in a functional ELISA with a linear range of 0.02-0.77 ng/mL. Cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D: ED50 = 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of Recombinant Mouse TNFa.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
CD120b, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFBR, TNFR-II, TNFR2, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Mouse TNFRSF1B Protein (C-terminal Human Fc Tag) (A331897)
ELISA examination of immobilized Recombinant Mouse TNF-a (2 µg/mL) binding Recombinant Mouse TNFRSF1B Protein (C-terminal Human Fc Tag) (A331897): linear range = 0.02-0.77 ng/mL
Recombinant Mouse TNFRSF1B Protein (C-terminal Human Fc Tag) (A331897)
TNFa-mediated cytotoxicityin L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D inhibited by Recombinant Mouse TNFRSF1B Protein (C-terminal Human Fc Tag) (A331897): ED50 = typically 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of Recombinant mouse TNFa.
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