Inhibition of cell proliferation of TF-1 human erythroleukemic cells: ED50 = 0.05-0.2ng/mL; Specific activity = 5.0×10^6-2.0×10^7units/mg. Stimulation of BMP7 expression in Rat fibroblast cells: 20ng/mL after 48h. Inhibition of the IL-4-dependent proliferation of HT-2 mouse T cells: ED50 = 0.78-3.1ng/mL; Specific activity = 3.23×10^5~1.28×10^6 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717)
SDS-PAGE of Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717) under reducing (R) and non-reducing (NR) conditions to show single bands at 15, 40-45, 55-60 kDa and 30, 80-90, 110-120kDa, respectively.
Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717)
Inhibition of cell proliferation for TF-1 Human erythroleukemic cells with Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717): ED50 = 0.05-0.2 ng/mL; Specific activity = 5.0×10^6-2.0×10^7units/mg.
Western Blot - Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717)
BMP7 expression in Rat fibroblast cells analyzed by western blot. Primary antibody incubation was performed with Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717) at 20 ng/mL after 48 hours and detected with chemiluminescence.
Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717)
Inhibition of IL-4-dependent cell proliferation for HT-2 mouse T cells with Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag) (A331717): ED50 = 0.78-3.1 ng/mL; Specific activity = 3.23×10^5~1.28×10^6 units/mg.
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