Recombinant Rat Leptin Triple Antagonist Protein (N-terminal PEG Tag)
Applications
SDS-PAGE
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Rat
Tag
N-terminal PEG Tag
Predicted MW
16 kDa
Biologically Active
Yes
Biological Activity
Leptin Antagonist Triple Mutant Rat Recombinant half-life in circulation after SC injection was over 20 hours.Leptin Antagonist Triple Mutant Rat Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Leptin Antagonist Triple Mutant Rat Recombinant in vitro activity is 5-6 fold lower than the non-pegylated antagonist, though in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.
Purity
>99% as determined by Gel Filtration and SDS-PAGE.
Product Form
Lyophilized
Concentration
0.65 mg/ml
Formulation
Lyophilized from a solution containing 3 µM Sodium Bicarbonate.
Storage
Shipped at ambient temperature. Lyophilized: Short term (several weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (several weeks) store at 4°C or 25°C. At lower concentrations it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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