Cell migration assay using Hela cells: 1ng/mL. Cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2: Specific activity = >1600 pmoles/min/µg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 50mM Tris, pH 7.5, with 10mM CaCl2 and 150mM NaCl (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758)
SDS-PAGE of Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758) under reducing (R) and non-reducing (NR) conditions to show single bands at 90-100 kDa and 180-200 kDa, respectively.
Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758)
Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758) induces cell migration of Hela cells at 1 ng/mL.
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