Cell migration assay using Hela cells: 1ng/mL. Cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2: Specific activity = >1600 pmoles/min/µg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 50mM Tris, pH 7.5, with 10mM CaCl2 and 150mM NaCl (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758)
SDS-PAGE of Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758) under reducing (R) and non-reducing (NR) conditions to show single bands at 90-100 kDa and 180-200 kDa, respectively.
Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758)
Recombinant Rat MMP9 Protein (C-terminal His Tag) (A331758) induces cell migration of Hela cells at 1 ng/mL.
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