Immobilized Rat VEGF164 (1 µg/mL) bind Human KDR in a functional ELISA with a linear range of 0.03-3.6 ng/mL. Cell proliferation assay using human umbilical vein endothelial cells (HUVEC): ED50 = typically 0.02-0.10 ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Rat VEGF-A Protein (C-terminal His Tag) (A331934)
SDS-PAGE of Recombinant Rat VEGF-A Protein (C-terminal His Tag) (A331934) under reducing (R) and non-reducing (NR) conditions to show single bands at 26-30 kDa and 45-60 kDa, respectively.
Standard Curve - Recombinant Rat VEGF-A Protein (C-terminal His Tag) (A331934)
ELISA examination of immobilized Recombinant Rat VEGF164 (1 µg/mL) binding Recombinant Rat VEGF-A Protein (C-terminal His Tag) (A331934): linear range = 0.03-3.6 ng/mL.
Recombinant Rat VEGF-A Protein (C-terminal His Tag) (A331934)
Cell proliferation assay using Recombinant Rat VEGF-A Protein (C-terminal His Tag) (A331934) on HUVEC Human umbilical vein endothelial cells : ED50 = typically 0.02-0.10 ng/mL.
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