Immobilized SARS-CoV-2 Spike RBD (2 µg/mL) bind Human ACE-2 in a functional ELISA with a linear range of 0.001-2.96 ng/mL. Immobilized human ACE2 on COOH Chip can bind SARS-COV-2 Spike RBD Protein in a SPR assay (Nicoya OpenSPR) with an affinity constant of 35.3 nM. Immobilized SARS-CoV-2 Spike RBD (2 µg/mL) bind Human ACE-2 in a functional ELISA with a linear range of 0.001-1.69 ng/mL.
Purity
>95% (by SDS-PAGE and HPLC).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant SARS-CoV-2 Spike RBD Protein (C-terminal His Tag) (A331946)
Recombinant SARS-CoV-2 Spike RBD Protein (C-terminal His Tag) (A331946) binding to immobilized human ACE2 on a COOH Chip, analyzed by surface plasmon resonance (SPR; Nicoya OpenSPR). Affinity constant = 35.3 nM.
Standard Curve - Recombinant SARS-CoV-2 Spike RBD Protein (C-terminal His Tag) (A331946)
ELISA examination of immobilized SARS-CoV-2 Spike RBD (2 µg/mL) binding Recombinant SARS-CoV-2 Spike RBD Protein (C-terminal His Tag) (A331946): linear range = 0.001-2.96 ng/mL.
Standard Curve - Recombinant SARS-CoV-2 Spike RBD Protein (C-terminal His Tag) (A331946)
ELISA examination of immobilized SARS-CoV-2 Spike RBD (2 µg/mL) binding Recombinant SARS-CoV-2 Spike RBD Protein (C-terminal His Tag) (A331946): linear range = 0.001-1.69 ng/mL.
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