97% of PCR product digestion after 30 minutes at 37°C. The incubation of 20 pmol of CAS9 protein with 1 µg pUC19 supercoiled vector at 37°C for 4 hours leads to formation of less than 10% open circular pUC19 form as determined by gel electrophoresis.
Purity
>90% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 10 mM Tris HCl Buffer, pH 7.5, with 300 mM Sodium Chloride, 100 µM EDTA, 50% Glycerol, and 1 mM DTT.
Storage
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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