Cloning of Restriction Fragments, Joining Linkers, Adapters to Blunt Ended DNA
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Enterobacteria Phage T4
Biologically Active
Yes
Unit Definition
1. One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 ul in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer. 2. One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmol of 32P from pyrophosphate to ATP, into Norit-adsorbable material in 20 minutes at 37°C. 3. 1 Weiss unit is equivalent to circa 67 cohesive-end ligation units.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 50 mM Tris HCl Buffer, pH 7.8, with 10 mM Magnesium Chloride, 10 mM DTT, 1 mM ATP, 25 µg/ml BSA, and DNA (0.1 to 1 µm in 5´ termini).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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