One unit is defined as the amount of enzyme required to cleave 3 µg of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.
Purity
>90% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 25 mM Tris Buffer, pH 8, with 75 mM Sodium Chloride, 5 mM EDTA, 10 mM GSH, and 50% Glycerol.
Storage
Shipped at 4°C. Short term (1 week) store at 4°C. Long term store below -18°C. Avoid freeze/thaw cycles.
General Notes
A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Recombinant TEV Protease (A63061)? Please let us know so that we can list the citation on this page.
