Synthetic Membrane Nanoparticle Human CD63 Protein
Description
Full-length transmembrane human CD63 protein expressed on the cell surface of membrane nanoparticles.
Applications
ELISA, FACS
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CD63 protein has a MW of 25.6 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Synthetic Membrane Nanoparticle Human CD63 Protein (A318464)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human CD63 Protein (A318464). Serial diluted Anti-CD63 Chimeric Antibody [DMC425] - Azide free (A318747) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CD63 Chimeric Antibody [DMC425] - Azide free (A318747) binding with CD63 full length membrane nanoparticles is 61.65 µg/ml.
Synthetic Membrane Nanoparticle Human CD63 Protein (A318464)
FACS analysis of Synthetic Membrane Nanoparticle Human CD63 Protein (A318464): A. Negative Control 1: Synthetic Membrane Nanoparticle Human CD63 Protein (A318464) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-CD63 Chimeric Antibody [DMC425] - Azide free (A318747) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human CD63 Protein (A318464) samples were stained with Anti-GPRC5D Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human CD63 Protein (A318464) samples were stained with Anti-CD63 Chimeric Antibody [DMC425] - Azide free (A318747) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
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