Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human AGTR1 protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Tag
N-terminal MBP Tag and C-terminal Flag Tag
Predicted MW
81.1 kDa
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 with 5% - 8% Trehalose. Do not use solvents with pH lower than 6.5 in subsequent experiments.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
AGTR1A, AGTR1B, Angiotensin II type-1 receptor, AT1 receptor, AT1AR, AT1BR, AT2R1, AT2R1B, Type-1 angiotensin II receptor
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immobilised Synthetic Nanodisc Human AGTR1 Protein at 0.2 µg/well can bind serial diluted Anti-Flag Monoclonal Antibody. After washing and incubation with a secondary antibody the EC50 for Synthetic Nanodisc Human AGTR1 Protein binding with Anti-Flag Monoclonal Antibody is 4.309ng/ml.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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Proteins predicted to interact with AGTR1
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
