Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human PAR2 protein to be purified and analysed in vitro.
Applications
Western Blot, ELISA
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Tag
C-terminal Flag Tag
Molecular Weight
Full length human PAR2 protein has a MW of 44.1 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with Synthetic Nanodisc Human PAR2 Protein (A318462) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with F2RL1-Nanodisc is 2.32 µg/ml.
Western Blot - Synthetic Nanodisc Human PAR2 Protein (A318462)
Western blot analysis of Synthetic Nanodisc Human PAR2 Protein (A318462) with Anti-Flag Tag Antibody at 1:5,000 dilution, followed by Goat Anti-Rabbit IgG Antibody (HRP) at 1:5,000 dilution.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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