Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human Smoothened protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Tag
C-terminal Flag Tag
Molecular Weight
Full length human Smoothened protein has a MW of 86.4 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
Protein Gx, Protein smoothened, SMO, SMOH
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Synthetic Nanodisc Human Smoothened Protein (A325295)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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Proteins predicted to interact with Smoothened
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
