This product recognizes the heavy and light chains of Chicken IgY.
Applications
ICC/IF, IHC, WB, Flow Cytometry
Recommended Dilutions
IF: 1-10 µg/mL, WB: 50-100 ng/mL, IHC: 1-10 µg/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate
CF®633
Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
2 mg/mL
Formulation
Liquid: Supplied in Phosphate Buffered Saline containing 50% glycerol, 2 mg/ml BSA, and 0.05% Sodium Azide
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes
Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.
Background
This secondary has been cross-adsorbed against bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Rat neuromuscular junction stained with CF®633 a-bungarotoxin to label acetylcholine receptors (magenta), with nuclei counterstained using DAPI (cyan).
This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
Relative photostability of CF®633 and Alexa Fluor® 647. Jurkat cells were stained with mouse anti-CD3 primary antibody followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Cells were continuously illuminated using a mercury arc lamp with a Cy®5 filter cube, and fluorescence intensity was monitored over time.
Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
CF®633 and CF®640R exhibit substantially greater photostability than Alexa Fluor® 647. Jurkat cells were stained with mouse anti-CD3 primary antibody followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Cells were continuously illuminated using a mercury arc lamp with a Cy®5 filter cube, with images acquired every 15 seconds for 5 minutes. Fluorescence intensity was normalized to the initial time point.
CF®633 produces the brightest far-red antibody conjugates. Jurkat cells were stained with mouse anti-CD3 or an isotype control antibody, followed by goat anti-mouse secondary antibodies conjugated with varying degrees of labeling (DOL, dye molecules per antibody). Fluorescence was measured in the APC detection channel on a BD FACSCalibur™ flow cytometer, and bars represent geometric mean fluorescence intensity.
Publishing research using Donkey Anti-Chicken IgY H&L Antibody (CF®633), Cross-Adsorbed (A343576)? Please let us know so that we can list the citation on this page.
