Donkey Anti-Mouse IgG H&L Antibody (CF®543), Cross-Adsorbed (A343771)

CF®543 produces substantially brighter antibody conjugates than Alexa Fluor® 546. Relative fluorescence intensity of goat anti-mouse secondary antibody conjugates is shown as a function of degree of labeling (DOL, dye molecules per antibody).

Normalized absorption and emission spectra of a CF®543 goat anti-mouse antibody conjugate measured in PBS.

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.