This product recognizes the heavy and light chains of Mouse IgG.
Applications
ICC/IF, IHC, WB, Flow Cytometry, ELISA
Recommended Dilutions
IF: 1-10 µg/mL, WB: 50-100 ng/mL, IHC: 1-10 µg/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate
CF®568
Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
2 mg/mL
Formulation
Liquid: Supplied in Phosphate Buffered Saline containing 50% glycerol, 2 mg/ml BSA, and 0.05% Sodium Azide. Lyophilized: Supplied in phosphate Buffered Saline with 15 mg/ml BSA and 20 mg/ml Trehalose.
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes
Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.
Background
This secondary has been cross-adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat and sheep serum.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
CF®568 exhibits greater photostability than Alexa Fluor® 568. Jurkat cells were stained with biotinylated anti-CD3 antibody followed by CF®568 or Alexa Fluor® 568 streptavidin conjugates. Cells were continuously illuminated using a mercury arc lamp with a Cy®3 filter cube. Images were acquired every 15 seconds for 5 minutes, and fluorescence intensity was normalized to the initial time point.
MCF-7 cells stained with a CF®568-conjugated monoclonal anti-Ep-CAM antibody (clone EGP40/826) to label Ep-CAM (red), with nuclei counterstained using Hoechst (blue).
This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
Publishing research using Donkey Anti-Mouse IgG H&L Antibody (CF®568), Cross-Adsorbed (A343519)? Please let us know so that we can list the citation on this page.
