This product recognizes the heavy and light chains of Mouse IgG.
Applications
ICC/IF, Flow Cytometry
Recommended Dilutions
IF: 2 drops/mL, Flow Cytometry: 1 drop/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate
CF®594
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 2 mg/mL BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes
Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Saccharomyces cerevisiae yeast stained with CF®594 wheat germ agglutinin to label cell walls (red) and CF®488A concanavalin A to label bud scars (green).
Photostability comparison of CF®594 and Alexa Fluor® 594. Jurkat cells were stained with mouse anti-CD3 primary antibody followed by CF®594 or Alexa Fluor® 594 goat anti-mouse IgG secondary antibodies. Cells were continuously illuminated, and fluorescence intensity was monitored over time.
Neuromuscular junctions in a rat skeletal muscle section stained with CF®594 a-bungarotoxin to label acetylcholine receptors (red), with nuclei counterstained using DAPI (blue).
CF®594 produces brighter antibody conjugates than Alexa Fluor® 594. Relative fluorescence intensity of goat anti-mouse IgG conjugates is shown as a function of degree of labeling (DOL, dye molecules per antibody).
Fixed oleic acid–treated HeLa cells stained with LipidSpot™ 488 to visualize lipid droplets (green), CF®594 wheat germ agglutinin to label the cell surface (red), and Hoechst 33258 to stain nuclei (blue).
This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
Publishing research using Donkey Anti-Mouse IgG H&L Antibody (CF®594), Cross-Adsorbed (A343761)? Please let us know so that we can list the citation on this page.
