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Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351)

Western Blot - Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351) - Antibodies.com Immunohistochemistry - Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351) - Antibodies.com
$105
Product Datasheet

Donkey anti-mouse IgG H&L (HRP) secondary antibody for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Donkey Anti-Mouse IgG H&L Antibody (HRP)
Description
Donkey anti-mouse IgG H&L (HRP) secondary antibody.
Host Species
Donkey
Target Species
Mouse
Applications
WB, IHC
Recommended Dilutions
WB: 1:10,000-1:30,000, IHC: 1:500-1:1,000
Immunogen
Mouse IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate

HRP

Product Form
Liquid
Concentration
Lot Specific
Purification
Affinity purification.
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.75% BSA.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351) - Antibodies.comEnlarge Image

Western Blot - Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351)

Western blot analysis of extracts of HeLa cells, using Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351) at 1:2000-1:30000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351) - Antibodies.comEnlarge Image

Immunohistochemistry - Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Donkey Anti-Mouse IgG H&L Antibody (HRP) (A17351) at a dilution of 1:20 - 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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