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Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434)

CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.com CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.com CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.com CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.com CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.com
$195
Product Datasheet

Donkey Anti-Rabbit IgG H&L (CF®488A) secondary antibody for ICC/IF, IHC, WB, Flow Cytometry and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.

  • Specifications

Name
Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed
Description
Donkey Anti-Rabbit IgG H&L (CF®488A) secondary antibody.
Host Species
Donkey
Target Species
Rabbit
Specificity
This product recognizes the heavy and light chains of Rabbit IgG.
Applications
ICC/IF, IHC, WB, Flow Cytometry, ELISA
Recommended Dilutions
IF: 1-10 µg/mL, WB: 50-100 ng/mL, IHC: 1-10 µg/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate

CF®488A

Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
2 mg/mL
Formulation
Liquid: Supplied in Phosphate Buffered Saline containing 50% glycerol, 2 mg/ml BSA, and 0.05% Sodium Azide. Lyophilized: Supplied in phosphate Buffered Saline with 15 mg/ml BSA and 20 mg/ml Trehalose.
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes

CF®488A is one of the most widely used green fluorescent dyes for biological imaging and flow cytometry. With excitation and emission maxima of 490/515 nm, it is compatible with standard 488 nm laser systems and serves as an excellent alternative to Alexa Fluor® 488 and FITC. CF®488A provides high signal intensity, outstanding photostability, and compatibility with advanced imaging techniques, making it ideal for demanding fluorescence applications requiring bright, stable labelling.

Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.

Background
This secondary has been cross-adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat and sheep serum.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.comEnlarge Image

Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434)

Normalized absorption and emission spectra of CF®488A goat anti-mouse IgG in PBS.
CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.comEnlarge Image

Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434)

HeLa cells stained with rabbit anti-COX IV followed by CF®488A goat anti-rabbit to label mitochondria (green), and mouse anti-Golgin 97 followed by CF®555 goat anti-mouse to label the Golgi apparatus (red). Actin filaments are visualized with CF®405 phalloidin (blue), and nuclei are counterstained with RedDot™2 (magenta).
CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.comEnlarge Image

Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434)

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
CF®488A - Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434) -Antibodies.comEnlarge Image

Donkey Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343434)

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
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