Donkey Anti-Rat IgG H&L Antibody (CF®750), Cross-Adsorbed (A344015)

CF®750 for in vivo small animal imaging. Tumor-bearing mice were imaged using an IVIS® imaging system (PerkinElmer) at 24 hours (left), 48 hours (center), and 96 hours (right) following intravenous administration of a CF®750–Avastin® conjugate. Image courtesy of Caliper Life Sciences.

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.

CF®750 demonstrates greater photostability than spectrally similar near-infrared dyes. Shown are the absorbance spectra of NIR fluorophores before light exposure (0 min, solid lines) and after 30 minutes of sunlight exposure (30 min, dashed lines). Following illumination, CF®750 retains higher absorbance compared to Cy®7 and Alexa Fluor® 750, indicating reduced photobleaching and superior resistance to photodegradation.

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.