Donkey Anti-Sheep IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343443)

CF®488A exhibits substantially greater photostability than FITC. HeLa cells were stained with either CF®488A phalloidin or FITC phalloidin and imaged at time zero or after 5 seconds of continuous excitation using a FITC filter set. FITC fluorescence photobleaches almost immediately, whereas CF®488A retains strong signal intensity, demonstrating its high photostability.

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.

Normalized absorption and emission spectra of CF®488A goat anti-mouse IgG in PBS.