This antibody recognises human IgE. Minimal cross-reactivity (<0.1% ) is expected with other human immunoglobulins and light chains.Goat anti Human IgE in a biotinylated format has been successfully employed as a detection reagent in the development of a sensitive sandwich ELISA for the measurement of human IgE.
Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Goat anti Human IgE polyclonal antibody recognizes human IgE. Minimal cross-reactivity (<0.1%) is expected with other human immunoglobulins and light chains.Goat anti Human IgE in a biotinylated format has been successfully employed as a detection reagent in the development of a sensitive sandwich ELISA for the measurement of human IgE.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Sandwich ELISA analysis of human IgE using Anti-Human IgE Antibody [E411 (5H2)] (A280306) as a capture reagent and Goat Anti-Human IgE Antibody (Biotin) (A282382) as a detection reagent with purified human IgE as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in geern and orange respectively.
Sandwich ELISA analysis of human IgE using Anti-Human IgE Antibody [E411 (5H2)] (A280306) as a capture reagent and Goat Anti-Human IgE Antibody (Biotin) (A282382) as a detection reagent with recombinant human IgE as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in geern and orange respectively.
Sandwich ELISA analysis of human IgE using Anti-Human IgE Antibody [E411 (5H2)] (A280306) as a capture reagent and Goat Anti-Human IgE Antibody (Biotin) (A282382) as a detection reagent with recombinant human IgE as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in geern and orange respectively.
Publishing research using Goat Anti-Human IgE Antibody (Biotin) (A282382)? Please let us know so that we can list the citation on this page.
