This antibody recognises Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins. This antibody is a performance tested, matched reagent for use with Bio-Rad HuCAL antibodies.
Applications
WB, ELISA
Recommended Dilutions
ELISA: 1:1,000 - 1:10,000, WB: 1:1,000 - 1:10,000
Immunogen
Human IgG F(ab')2.
Clonality
Polyclonal
Isotype
IgG
Conjugate
HRP
Product Form
Liquid
Concentration
500 µg/ml
Purification
Affinity chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 0.01% Thiomersal and 1% BSA.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins. This antibody is a performance tested, matched reagent for use with Bio-Rad HuCAL antibodies.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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