Goat Anti-Mouse IgG Fc Antibody (CF®770), Cross-Adsorbed
Description
Goat Anti-Mouse IgG Fc (CF®770) secondary antibody.
Host Species
Goat
Target Species
Mouse
Specificity
This goat anti-mouse IgG3 secondary antibody was raised against mouse IgG3 and specifically recognizes the Fc region of the mouse IgG3 heavy chain.
Applications
ICC/IF, IHC, WB, Flow Cytometry
Recommended Dilutions
IF: 1-10 µg/mL, WB: 50-100 ng/mL, IHC: 1-10 µg/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate
CF®770
Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
2 mg/mL
Purification
The antibody is affinity-purified and cross-adsorbed.
Formulation
Liquid: Supplied in phosphate buffered saline (PBS) containing 50% glycerol, 2 mg/ml BSA, and 0.05% sodium azide. Lyophilized: Supplied in phosphate Buffered Saline with 15 mg/ml BSA and 20 mg/ml trehalose.
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes
Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Goat Anti-Mouse IgG Fc Antibody (CF®770), Cross-Adsorbed (A344089)
CF®680 provides brighter signal than IRDye® 680LT for near-infrared western blot detection. Two-fold dilutions of HeLa cell lysate (2 µg to 0.125 µg, lanes 1–5) were resolved and transferred to PVDF membranes. Mouse anti-tubulin and rabbit anti-COX IV primary antibodies were detected using IRDye® 680LT or CF®680 anti-mouse secondary antibodies (red) and IRDye® 800CW or CF®770 anti-rabbit secondary antibodies (green). Membranes were imaged on a LI-COR® Odyssey® NIR imaging system. Band quantification revealed approximately 50% higher signal intensity with CF® dyes compared to IRDye®. M indicates the molecular weight marker.
Goat Anti-Mouse IgG Fc Antibody (CF®770), Cross-Adsorbed (A344089)
Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
Goat Anti-Mouse IgG Fc Antibody (CF®770), Cross-Adsorbed (A344089)
This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
Goat Anti-Mouse IgG Fc Antibody (CF®770), Cross-Adsorbed (A344089)
Near-IR CF® dyes provide superior brightness and photostability compared with conventional near-infrared dyes, with improved solubility and labeling performance. For microscopy, higher concentrations of CF®750 and CF®770 conjugates may be required due to reduced detector sensitivity at longer wavelengths.
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