This antibody recognises mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues. Goat IgG shows minimal binding to Fc receptors on rat tissue. We recommend diluting this product in buffer containing 10% normal rat serum to remove any residual cross-reactivity.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:50 - 1:200, Use 50ul of the suggested working dilution to label 106 cells in 100µl.
Immunogen
Mouse IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Product Form
Liquid
Concentration
500 µg/ml
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Goat anti Mouse IgG antibody recognizes mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues. Goat IgG shows minimal binding to Fc receptors on rat tissue. We recommend diluting this product in buffer containing 10% normal rat serum to remove any residual cross-reactivity.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow cytometric analysis of Mouse anti Rat MHC Class I RT1Ac (A281430) in a flow cytometric analysis of BN rat spleen cells using Goat Anti-Mouse IgG Antibody (FITC), Cross-Adsorbed (A282488).
Flow cytometric analysis of Anti-SLA Class II DR Antibody [2E9/13] (A279210) in a flow cytometric analysis of porcine peripheral blood monocytes using Goat Anti-Mouse IgG Antibody (FITC), Cross-Adsorbed (A282488)
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