This antibody recognises mouse IgG2a. This antibody has been cross absorbed against mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2a antibody shows minimal cross-reactivity with human immunoglobulins.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:500, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Immunogen
IgG2a paraproteins from BALB/c mice.
Clonality
Polyclonal
Isotype
IgG
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Product Form
Liquid
Concentration
500 µg/ml
Formulation
Supplied in Phosphate Buffered Saline with Sucrose and <0.1% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure A. Anti-CD6 / T12 Antibody [MIL8] (A281211) detected with Goat Anti-Mouse IgG2a Antibody (PE) (A282650) and Mouse IgG2b (FITC) - Isotype Control (A282451). Figure B. Anti-CD6 / T12 Antibody [MIL8] (A281211) detected with Goat Anti-Mouse IgG2a Antibody (PE) (A282650) and Anti-CD45 Antibody [K252.1E4] (FITC) (A280792). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Figure A. Anti-CD45 Antibody [K252.1E4] (FITC) (A280792) and Mouse IgG2a - Isotype Control (A284023) detected with Goat Anti-Mouse IgG2a Antibody (PE) (A282650). Figure B. Anti-CD45 Antibody [K252.1E4] (FITC) (A280792) and Anti-CD6 / T12 Antibody [MIL8] (A281211) detected with Goat Anti-Mouse IgG2a Antibody (PE) (A282650). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Figure A. Anti-CD6 / T12 Antibody [MIL8] (A281211) detected with Goat Anti-Mouse IgG2a Antibody (PE) (A282650) and Mouse IgG2b (FITC) - Isotype Control (A282451). Figure B. Anti-CD6 / T12 Antibody [MIL8] (A281211) detected with Goat Anti-Mouse IgG2a Antibody (PE) (A282650) and Anti-CD45 Antibody [K252.1E4] (FITC) (A280792). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
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