Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438)

CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.com CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.com CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.com CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.com CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.com
$195
Product Datasheet

Goat Anti-Rabbit IgG H&L (CF®488A) secondary antibody for ICC/IF, IHC, WB, Flow Cytometry and ELISA.

100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.

  • Specifications

Name
Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed
Description
Goat Anti-Rabbit IgG H&L (CF®488A) secondary antibody.
Host Species
Goat
Target Species
Rabbit
Specificity
This product recognizes the heavy and light chains of Rabbit IgG.
Applications
ICC/IF, IHC, WB, Flow Cytometry, ELISA
Recommended Dilutions
IF: 1-10 µg/mL, WB: 50-100 ng/mL, IHC: 1-10 µg/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate

CF®488A

Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
2 mg/mL
Formulation
Liquid: Supplied in Phosphate Buffered Saline containing 50% glycerol, 2 mg/ml BSA, and 0.05% Sodium Azide. Lyophilized: Supplied in phosphate Buffered Saline with 20 mg/ml BSA and 20 mg/ml Trehalose.
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes

Goat Anti-Rabbit IgG H&L Antibody (CF®488A) is a great alternative to goat anti-rabbit Alexa Fluor® 488, delivering bright fluorescence, excellent photostability, and strong performance in fluorescence microscopy, flow cytometry, and other fluorescence-based applications. CF®488A offers spectral properties comparable to Alexa Fluor® 488 while supporting advanced imaging techniques including STED, STORM, 2-photon microscopy, TIRF, and DNA-PAINT.

Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.

Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.comEnlarge Image

Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438)

HeLa cells stained with rabbit anti-COX IV followed by CF®488A goat anti-rabbit to label mitochondria (green), and mouse anti-Golgin 97 followed by CF®555 goat anti-mouse to label the Golgi apparatus (red). Actin filaments are visualized with CF®405 phalloidin (blue), and nuclei are counterstained with RedDot™2 (magenta).
CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.comEnlarge Image

Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438)

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.comEnlarge Image

Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438)

CF®488A exhibits substantially greater photostability than FITC. HeLa cells were stained with either CF®488A phalloidin or FITC phalloidin and imaged at time zero or after 5 seconds of continuous excitation using a FITC filter set. FITC fluorescence photobleaches almost immediately, whereas CF®488A retains strong signal intensity, demonstrating its high photostability.
CF®488A - Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438) -Antibodies.comEnlarge Image

Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438)

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
Publishing research using Goat Anti-Rabbit IgG H&L Antibody (CF®488A), Cross-Adsorbed (A343438)? Please let us know so that we can list the citation on this page.