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Goat Anti-Rat IgG H&L Antibody (FITC) (A17330)

Western Blot - Goat Anti-Rat IgG H&L Antibody (FITC) (A17330) - Antibodies.com
$105
Product Datasheet

Goat anti-rat IgG H&L (FITC) secondary antibody for ICC/IF and Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Goat Anti-Rat IgG H&L Antibody (FITC)
Description
Goat anti-rat IgG H&L (FITC) secondary antibody.
Host Species
Goat
Target Species
Rat
Applications
ICC/IF, Flow Cytometry
Recommended Dilutions
ICC/IF: 1:50-1:200, Flow Cytometry: 1:50-1:200
Immunogen
Rat IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Product Form
Liquid
Concentration
Lot Specific
Purification
Affinity purification.
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.75% BSA, and 0.025% Sodium Azide.
Storage
Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Goat Anti-Rat IgG H&L Antibody (FITC) (A17330) - Antibodies.comEnlarge Image

Western Blot - Goat Anti-Rat IgG H&L Antibody (FITC) (A17330)

Western blot analysis of extracts of A-431 cells, using Goat Anti-Rat IgG H&L Antibody (FITC) (A17330) at 1:1,000 dilution or EGFR antibody (A11351). A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
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