Goat F(ab')2 Anti-Mouse IgG H&L Antibody (CF®640R), Cross-Adsorbed (A343888)

CF®640R exhibits substantially greater photostability than Alexa Fluor® 647. Jurkat cells were stained with mouse anti-CD3 primary antibody followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Cells were continuously illuminated using a mercury arc lamp with a Cy®5 filter cube, with images acquired every 15 seconds for 5 minutes. Fluorescence intensity was normalized to the initial time point.

Normalized absorption and emission spectra of a CF®640R goat anti-mouse antibody conjugate measured in PBS.

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.

CF®640R produces brighter antibody conjugates than other far-red dyes. Jurkat cells were stained with mouse anti-CD3 primary antibody or without primary antibody as a control, followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Fluorescence was analyzed on a BD FACSCalibur™ flow cytometer using the FL4 detection channel, and bars represent geometric mean fluorescence intensity.