This antibody recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:25 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Immunogen
Rat IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
FITC conjugated Goat (F(ab′)2 anti Rat IgG antibody recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Goat F(ab')2 Anti-Rat IgG Antibody (FITC), Cross-Adsorbed (A282487)? Please let us know so that we can list the citation on this page.
