After reconstitution; phosphate Buffered Saline with 15 mg/ml BSA and 20 mg/ml trehalose.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes
Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Llama Anti-Rabbit IgG + Rat IgG Antibody (CF®488A) (A343909)
CF®488A exhibits substantially greater photostability than FITC. HeLa cells were stained with either CF®488A phalloidin or FITC phalloidin and imaged at time zero or after 5 seconds of continuous excitation using a FITC filter set. FITC fluorescence photobleaches almost immediately, whereas CF®488A retains strong signal intensity, demonstrating its high photostability.
Llama Anti-Rabbit IgG + Rat IgG Antibody (CF®488A) (A343909)
This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
Llama Anti-Rabbit IgG + Rat IgG Antibody (CF®488A) (A343909)
HeLa cells stained with rabbit anti-COX IV followed by CF®488A goat anti-rabbit to label mitochondria (green), and mouse anti-Golgin 97 followed by CF®555 goat anti-mouse to label the Golgi apparatus (red). Actin filaments are visualized with CF®405 phalloidin (blue), and nuclei are counterstained with RedDot™2 (magenta).
Llama Anti-Rabbit IgG + Rat IgG Antibody (CF®488A) (A343909)
Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
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