Anti-Caveolin-2 Antibody [ARC0323] (A307368)

Name: Anti-Caveolin-2 Antibody [ARC0323]
Brand: Antibodies.com
SKU: A307368
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0323] antibody to Caveolin-2 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Caveolae Marker Endosomal Marker

Specifications

Name

Anti-Caveolin-2 Antibody [ARC0323]

Description

Rabbit monoclonal [ARC0323] antibody to Caveolin-2.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Caveolin-2 (P51636).

Sequence

MGLETEKADVQLFMDDDSYSHHSGLEYADPEKFADSDQDRDPHRLNSHLKLGFEDVIAEPVTTHSFDKVWICSHALFEISKYVMYKFLTVFLAIPLAFIA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0323

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CAV, CAV2, CAV2_HUMAN, Caveolae protein 20 kD, Caveolin 2, Caveolin 2 isoform a and b, MGC12294, OTTHUMP00000025032, OTTHUMP00000195982

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Caveolin-2 Antibody [ARC0323] (A307368)

Western blot analysis of extracts of Mouse lung, using Anti-Caveolin-2 Antibody [ARC0323] (A307368) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Caveolin-2 Antibody [ARC0323] (A307368)

Western blot analysis of extracts of 293T cells, using Anti-Caveolin-2 Antibody [ARC0323] (A307368) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Caveolin-2 Antibody [ARC0323] (A307368)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Caveolin-2 Antibody [ARC0323] (A307368) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-Caveolin-2 Antibody [ARC0323] (A307368)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Caveolin-2 Antibody [ARC0323] (A307368). This Western blot was performed on the immunoprecipitate using Anti-Caveolin-2 Antibody [ARC0323] (A307368) at a dilution of 1:1000.

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