Anti-CBX4 Antibody (A11936)

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This product is discontinued

Anti-CBX4 Antibody (A11936) has been discontinued and is no longer available.

View all CBX4 Antibodies.

Specifications

Name

Anti-CBX4 Antibody

Description

Rabbit polyclonal antibody to CBX4.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2000, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2).

Sequence

AVGLNLSHVRKRCLSETHGEREPCKKRLTARSISTPTCLGGSPAAERPADLPPAAALPQPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, hPc2, Pc2, Polycomb 2 homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-CBX4 Antibody (A11936)

Western blot analysis of extracts of various cell lines, using Anti-CBX4 Antibody (A6221) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

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Immunohistochemistry - Anti-CBX4 Antibody (A11936)

Immunohistochemistry of paraffin-embedded human lung cancer using Anti-CBX4 Antibody (A6221) at dilution of 1:100 (40x lens).

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Immunoprecipitation - Anti-CBX4 Antibody (A11936)

Immunoprecipitation analysis of 200µg extracts of HepG2 cells using 1µg Anti-CBX4 Antibody (A6221). Western blot was performed from the immunoprecipitate using Anti-CBX4 Antibody (A6221) at a dilition of 1:500. ___Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.