CBX5 Cell Based ELISA Kit (A103513)

CBX5 Cell Based ELISA Kit

Cell-based (qualitative)

This CBX5 Cell Based ELISA Kit allows for the detection of CBX5 and the effects that certain stimulation conditions have on CBX5 expression in different cell lines. Qualitative determination of CBX5 concentration is achieved by an indirect ELISA format. In essence, the CBX5 is captured by Anti-CBX5 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CBX5 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

Antigen p25, CBX5_HUMAN, CG8409, Chromobox 5, Chromobox homolog 5, Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Chromobox protein homolog 5, Epididymis luminal protein 25, HEL25, Heterochromatin protein 1, Heterochromatin protein 1 alpha, Heterochromatin protein 1 homolog alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1Hs alpha, Su(var)205