Anti-CCR9 Antibody [9B1] (PE) (A279562)

Anti-CCR9 Antibody [9B1] (PE)

Rat monoclonal [9B1] antibody to CCR9 (PE).

This antibody recognises mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9. CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.Clone 9B1 blocks CCL25-induced Ca2+ release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Mouse

A peptide corresponding to 25 amino acids from the N-terminus of murine CCR9, conjugated to Keyhole Limpet Haemocyanin.

Rat

Monoclonal

9B1

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD199 antibody, clone 9B1 detects mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9. CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.Clone 9B1 blocks CCL25-induced Ca2+ release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages.

C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, CDw199, G-protein coupled receptor 28, GPR-9-6, GPR28

This product is for research use only. It is not intended for diagnostic or therapeutic use.