Anti-CD11b Antibody [M1/70.15] (A280992)

Anti-CD11b Antibody [M1/70.15]

Rat monoclonal [M1/70.15] antibody to CD11b.

This antibody recognises the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

IHC-P, IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:50 Pack Size: 0.25 mg 1:200 Pack Size: 0.1 mg

Mouse, Human, Rabbit

T cell enriched splenocytes from B10 mice.

Rat

Monoclonal

M1/70.15

IgG2b

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995). This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

antigen CD11b (p170), Antigen CD11b p170, CD11 antigen like family member B, CD11 antigen-like family member B, CD11b/CD18, CD49d, Cell surface glycoprotein MAC-1 subunit alpha, Complement component 3 receptor 3 subunit, Complement Component Receptor 3 Alpha, Complement receptor type 3, alpha subunit, CR 3 alpha chain, CR 3 alpha chain (CR3A), CR-3 alpha chain, CR3, CR3A, F730045J24Rik, Integrin Alpha M, Integrin alpha M chain, Integrin alpha-M, Integrin beta 2 alpha subunit, Integrin subunit alpha M, integrin, alpha M (complement component 3 receptor 3 subunit), ITAM_HUMAN, ITGAM, Leukocyte adhesion receptor MO1, Ly-40, MAC 1, Mac-1a, MAC1, Mac1, alpha subunit, MAC1A, Macrophage antigen alpha polypeptide, MGC117044, Mo1, alpha subunit, MO1A, Neutrophil adherence receptor, Neutrophil adherence receptor alpha M subunit, SLEB6