Anti-CD11b Antibody [OX-42] (A281462)

Anti-CD11b Antibody [OX-42]

Mouse monoclonal [OX-42] antibody to CD11b.

This antibody recognises rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain. Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes as well as inhibit myelin binding and uptake.

IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Resident rat peritoneal macrophages.

Mouse

Monoclonal

OX-42

IgG2a

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat CD11b, clone OX-42 recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain (Robinson et al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinson et al.1986) as well as inhibit myelin binding and uptake (van der Laan et al.1996).

antigen CD11b (p170), Antigen CD11b p170, CD11 antigen like family member B, CD11 antigen-like family member B, CD11b/CD18, CD49d, Cell surface glycoprotein MAC-1 subunit alpha, Complement component 3 receptor 3 subunit, Complement Component Receptor 3 Alpha, Complement receptor type 3, alpha subunit, CR 3 alpha chain, CR 3 alpha chain (CR3A), CR-3 alpha chain, CR3, CR3A, F730045J24Rik, Integrin Alpha M, Integrin alpha M chain, Integrin alpha-M, Integrin beta 2 alpha subunit, Integrin subunit alpha M, integrin, alpha M (complement component 3 receptor 3 subunit), ITAM_HUMAN, ITGAM, Leukocyte adhesion receptor MO1, Ly-40, MAC 1, Mac-1a, MAC1, Mac1, alpha subunit, MAC1A, Macrophage antigen alpha polypeptide, MGC117044, Mo1, alpha subunit, MO1A, Neutrophil adherence receptor, Neutrophil adherence receptor alpha M subunit, SLEB6